Beijing HiYi Technology Co., Ltd
Beijing HiYi Technology Co., Ltd
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Laboratory Equipment 96 wells Real Time Quantitative Accurate 96 Real-time PCR Machine
Laboratory Equipment 96 wells Real Time Quantitative Accurate 96
Real-time PCR Machine
As a necessary choice for quantitative analysis of molecular biology, real-time PCR system has been widely used in various fields such as scientific research, clinical detection and diagnosis, quality and safety testing, and forensic applications.
The Accurate 96 real-time fluorescent quantitative PCR system is based on the product design concept and manufacturing process of the global field of vision. Fluorescence and multi-color crosstalk between multiple channels and multiple samples, detection sensitivity, temperature uniformity and accuracy have reached international advanced level, supporting the application of all common QPCR detection modes.
· Up to 6 fluorescence detection channels allowing multiplex PCR.
· Effectively reduce multi-color crosstalk and edge effect, no ROX correction required.
· New optical scanning detection system
· Innovative scanning method and time-resolved signal separation technology
· Unique edge temperature compensation technology to minimize "edge effect".
· User-friendly software
· Innovation technology with long-lasting LED light provides reliability results.
| Temperature control system | |
| Sample capacity | 0.1ml PCR tubes×96, 8×12 PCR plate or 96 well plate ×1 |
| Reaction volume | 10-50 μl |
| Thermal cycle technology | Peltier |
| Max. Heating/Cooling rate | 6.0° C/s |
| Heating temperature range | 4 – 100 °C |
| Temperature accuracy | ± 0.2°C |
| Temperature uniformity | ±0.2℃ @60℃ , ±0.3℃ @95℃ |
| Temperature gradient setting range | 30–100°C |
| Temperature gradient difference setting range | 1 – 36°C |
| Detection system | |
| Excitation light source | 4/6 monochrome high efficiency LEDs |
| Detection device | PMT |
| Detection mode | Time-resolved signal separating technology |
| Excitation/detection wavelength range | 455-650nm/510-715nm |
| Fluorescent channels | 4/6 channels |
| Supported dye | FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
| Sensitivity | Single copy gene |
| Resolution | 1.33 folds copy number difference can be distinguished in single-plex qPCR |
| Dynamic range | 10 orders of magnitude copies |